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The two protein bands from 40 kDa to thirty kDa had been absent just after 24 h incubation at 45 °C. For the rest of the bands, many concentrations of all three proteases retained protein bands comparable to their initial molar masses (Figure 6a,b). The presence of bands at low molecular pounds in fish roe just before hydrolysis was unanticipated. Within the report by Ghelichi et al. [eight], minimal peaks of a comparatively small molecular pounds array (three–fourteen kDa) ended up noticed in defatted carp roe homogenate, possibly on account of an autolytic approach developing in fish roe and/or the defatting approach leading to the formation of minimal-molecular-body weight peptides.
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